FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23622554
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG132600.056.42835694
CAAGTCG100650.030.86636794
TAAGTCG36200.026.8756994
GAAGTCG144800.021.13052494
AAAGTCG49850.016.87662994
GTTTTTT320600.016.0673731
TTTTTTC383450.014.9291942
GTTTAAT99800.013.6573141
CGTGATA24050.013.4844069
CGAAAAT39400.013.1218281
TACGCTA9050.012.9834259
TTTTTTG421500.012.7786472
TTAGCGA27050.012.5101657
CGGAAAT53750.012.5041861
GTTTTCT222900.012.4405561
AAAAATC213550.012.2369462
TAGCGAG28450.011.8945518
CGCAATT19400.011.8711341
TATACGC8900.011.6179777
GGAAAAT173150.011.6176721
Produced by FastQC (version 0.11.5)