FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23024494
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106150.053.7522494
CAAGTCG74950.027.27818794
TAAGTCG35600.022.9719194
GAAGTCG120350.020.42459594
GCGAAAT30650.014.874391
GTTTTTT311250.014.8587971
AAAGTCG53200.014.84210794
GTTTAAT106150.014.301461
TTTTTTC402600.014.1840532
CGGATAT25700.014.0817131
AAATCCA219900.013.8071864
GTTTTAT176300.013.4095311
TATACGC9600.013.2187527
TTTTTTG445250.013.0681652
TACGCTA8850.012.7457639
AAATCGA35800.012.7346384
AATCCAC122300.012.2976285
GAAAAAT268300.012.2974291
CGAAAAT43750.012.2468571
GTCGATT20050.012.1895271
Produced by FastQC (version 0.11.5)