FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23024494
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134100.042.443794
CAAGTCG96400.024.96265694
TAAGTCG34850.019.2855194
GAAGTCG119600.017.9590394
GTTTTTT336650.015.3432341
GCGAAAT26150.014.738051
AAAGTCG57650.014.59323694
TACGCTA10350.014.5314039
TATACGC11250.013.7866677
CGCAAAT29450.013.2461811
CGCGTTT16750.013.188061
AAATCCA218750.013.0203434
CGGATAT26050.012.9904031
CGAAAAT48150.012.98234751
CGCAATT21000.012.9809531
CGAATCG14900.012.9328873
AAATCGA33400.012.3832344
CTTAGAT76700.012.3780971
CGAAGAT82350.012.3278681
AAAAATC236100.012.3223222
Produced by FastQC (version 0.11.5)