FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21373574
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT299400.015.0544411
CGAATCG16150.013.6780183
CGAAAAC28450.013.5465731
CGAAAAT49250.013.4558371
CGCGATT6451.4551915E-1113.1162781
CGCAAAC39850.013.0915941
TCGATTG20900.012.8181812
CTCGATT35200.012.8181811
AAATCGA34250.012.7620424
CGGATAT20050.012.6583531
CGCGAAC12750.012.5333341
GCCGATT24100.012.0912861
CTCAGAT164850.012.0315431
TCGAATC20050.011.95511152
CTTTAAC78150.011.8477281
CGTGATA25000.011.8449
GTTTAAT90900.011.7370741
GGAAAAT183850.011.7340211
CTCGGAT65350.011.5791871
CGCAAAT29700.011.5521881
Produced by FastQC (version 0.11.5)