FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24433981
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG185250.049.9557394
CAAGTCG138900.027.71274294
TAAGTCG43300.025.83371594
GAAGTCG162300.023.4855294
AAAGTCG66850.016.94390394
GTCGGAT53600.014.73134294
TCGGATC53250.013.85727794
CGAAAAT46750.013.7732621
GTTTTTT316550.013.7191591
CGCGTAT4659.1556285E-712.1290321
CGCAAAT34500.012.1246371
CGAAAAC37300.012.0965141
CTCGATT45250.011.7370151
CGGAAAT46650.011.586281
TCGATTG25750.011.4990292
TTAGCGA19250.011.2311687
GTTTTAC77900.011.1617451
GCGAAAT30700.011.02281
GTTTTAT156400.010.998721
CGAAGAT89000.010.931461
Produced by FastQC (version 0.11.5)