FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22454653
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG121100.054.10239494
CAAGTCG99000.028.01010194
TAAGTCG34750.025.15683694
GAAGTCG138450.021.183194
TATACGC7450.016.4026857
AAAGTCG48850.014.91299994
CGAAAAT33150.014.1779791
GTTTATT97650.013.5248351
GTTTTTT275300.012.479841
GTAAAAC64550.012.3780021
TACGCTA9200.012.2608719
CGCAAAT31300.011.862621
ATATACG9150.011.8142096
CGCAATT23100.011.8008671
CTTTATT155850.011.5200521
AATCGGG52650.011.2478655
GTCGGTT49800.011.2309251
GTTTAAT92600.011.2170631
CGAAAAC32050.011.1450851
TTAGCGA29300.011.068267
Produced by FastQC (version 0.11.5)