FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22121208
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG115350.052.1950694
CAAGTCG90250.027.80941694
TAAGTCG29000.025.93103494
GAAGTCG126900.019.81481694
AAAGTCG48500.015.21443394
CGCAATT22050.013.6417241
TTAGCGA25000.013.3487
GTCGGTT47400.013.1877631
ATTAGCG7401.8189894E-1212.7027036
CGAAAAT34250.012.6248171
TATACGC7451.8189894E-1212.617457
CGGAAAT47150.012.4602341
AATCGGG51500.012.0466015
TACGCTA7451.4551915E-1111.9865779
CGAATCG13450.011.8810413
GTTTAAT86350.011.64794351
CGCGAAT7702.7284841E-1111.5974031
TAGCGAG29000.011.5068978
GGAAAAT154050.011.502111
GTTTATT94850.011.4464941
Produced by FastQC (version 0.11.5)