FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22844922
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG102250.054.37750694
CAAGTCG88100.029.76844694
TAAGTCG30000.023.81333494
GAAGTCG110900.018.7745794
AAAGTCG42400.014.410377594
ATCGTAT18100.012.7237576
CGCGTAT4506.1569517E-712.5333331
TTAGCGA18850.011.968178
AATCGTA23650.011.3276955
TCGTATC19650.011.2417317
CGTATCG17650.011.1841363
CGGGAAT52450.011.1115351
TAGCGAG22700.010.766529
GGCGAAT53200.010.6015031
GTCGGAT27100.010.5793361
CGCGTAA7754.3728505E-910.3096771
GGGAAAT167050.010.2975161
GTTCGAT28950.010.227981
GTCGTTT19450.010.1491011
CGGAAAT42450.010.0753831
Produced by FastQC (version 0.11.5)