FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22844922
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127750.043.44970794
CAAGTCG103550.027.05166894
TAAGTCG29450.021.54499294
GAAGTCG114350.017.67380794
AATCGTA21950.015.630985
GCGGTAT24800.014.0241941
TTAGCGA19200.013.218758
ATCGTAT17800.012.9382016
CGCGTAA7055.456968E-1212.6666671
TAGCGAG23550.011.7749479
AAAGTCG51500.011.68155394
GGCGATT36250.011.4096561
GTTCGAT28800.011.0972211
CGCGGAT25850.010.9090911
TCGTATC20300.010.6502467
CGGTATA13850.010.5198562
CGGGAAT52400.010.4942751
GGCGAAT44300.010.0790061
CGCGTAT4701.6460632E-410.01
GCCGGTT56850.09.8381711
Produced by FastQC (version 0.11.5)