FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22561671
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG121000.055.66198394
CAAGTCG86600.029.46997894
TAAGTCG38050.025.44546994
GAAGTCG145050.019.08514494
GTCGGTT46700.016.5053541
AAAGTCG51700.015.45454794
TTAGCGA20850.014.8776987
CGGAAAT47000.014.61
CGCGATT7700.012.8181821
CGAATAT13750.012.6472731
CGAAAAT35850.012.192471
CGCGTAT4254.445794E-612.1647051
CGAATCG15250.011.7114763
CGCGAAC15350.011.63517951
GTCGGGT53850.011.5208921
GGAAAAT182100.011.4854481
GTTTAAT87200.011.4266061
TAGCGAG27300.011.1904768
CGAAGAT98950.010.924711
AATCGGG42650.010.7995325
Produced by FastQC (version 0.11.5)