FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21995763
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG102850.032.35391294
CAAGTCG80800.017.97400994
TAAGTCG28900.016.26297694
GTTTTTT274900.015.0625681
CTTAGAT66650.014.1035251
CGAAAAC39050.013.8412281
GAAGTCG102350.013.776257594
CTCGATT44850.013.7279821
TACGCTA8250.013.6727269
CGAATCG15800.013.3860753
GTTTAAT87950.012.9857861
CGCAATT21800.012.9357781
CGCGATT7750.012.7354831
AAATCCA193600.012.5511364
AAATCGA31200.012.5032044
CGGAAAT44800.012.4843751
CGCAAAC44450.012.3712031
AATCCAA109750.012.3334855
CTCAGAT173250.012.3163051
GTTTTAC71800.012.3064061
Produced by FastQC (version 0.11.5)