FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21697363
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126250.054.27801594
CAAGTCG98200.028.2861594
TAAGTCG34000.027.78529294
GAAGTCG141050.021.7922794
CGAAAAT32800.015.4756091
AAAGTCG49250.014.69644694
GTTTTTT269800.014.3891771
TTTTTTC333150.013.839712
CGGAAAT43650.012.3825881
TTTTTTG365900.012.0743372
CGCAAAT28950.011.8514681
GAAAAAT190200.011.8364881
CGCAAAC43050.011.7909391
GTTTTAT139600.011.6153291
GCGGTAT24950.011.4909821
AAAAATC182900.011.4866052
GTCGGAT32050.011.4383771
GCGAAAT30100.011.3986711
GTTTAAT76350.011.3883421
GGAAAAT151300.011.3694641
Produced by FastQC (version 0.11.5)