FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21994875
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG85150.045.6476894
CAAGTCG67000.023.7104594
TAAGTCG26500.022.34717294
GAAGTCG104350.017.29563994
GTTTTTT282500.016.3044261
TTTTTTC399200.015.6117252
TTTTTTG429550.014.6180892
CGAATCG14350.014.411153
GTTTAAT91400.013.5240711
CTCGATT41400.013.5096631
AAAGTCG45850.013.12104894
TTTTTTA404350.013.0881662
GAAAAAT215400.012.9828231
CTTAGAT68250.012.9465211
CGAAAAT39000.012.8948731
AAAAATC216650.012.7777532
CGAATAT12200.012.7131151
AAATCCA194900.012.6121094
GTCGATT19850.012.5491181
TCGATTG18000.012.5333332
Produced by FastQC (version 0.11.5)