FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21994875
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114900.033.6649394
CAAGTCG85700.020.07234494
TAAGTCG26000.018.9807794
GAAGTCG106850.016.8909794
GTTTTTT298100.015.4827251
CGAAAAC36450.015.3443081
CTCGATT42750.014.8421061
GCGAAAT25750.014.7844671
CGGATAT21050.014.7363431
AAATCGA29500.014.1796634
TATACGC8650.014.1271697
TACGCTA8750.013.9657149
CGCAAAT30600.013.823531
AATCGTA18350.013.3188025
CGAAAAT44850.013.099221
CTTAGAT71250.012.6652631
AAATCCA195450.012.6247124
CGCAAAC41300.012.5181611
CGAATCG15050.012.4916953
CGCAATT20400.012.4411781
Produced by FastQC (version 0.11.5)