FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23090074
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG93950.049.37626694
CAAGTCG74350.025.34902694
TAAGTCG27750.019.1387494
GAAGTCG111950.018.09468794
AAAGTCG44100.013.00226894
GTCGGTT47850.012.8672931
GTTCGAT32550.012.5622121
CGGGAAT54450.011.3076221
TTCGATT34100.011.0263932
GGCGAAT52700.010.6129051
GGCGGTT61550.010.3850541
CGCGTAA10305.456968E-1210.0388361
CTCGGTT37500.010.0266671
GTTTATT107450.09.9292711
GGCGATT40100.09.6109721
CGGTTGT57450.09.4081823
AATCGTA20750.09.2867475
TTAGCGA20300.09.2610858
GGAAAAT179400.09.1432561
CGCAATT22150.09.1241541
Produced by FastQC (version 0.11.5)