FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22337257
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG113350.054.56727294
CAAGTCG88750.028.80901594
TAAGTCG34450.025.37590894
GAAGTCG132400.021.192694
AAAGTCG45700.016.04376494
TATACGC7100.015.8873247
TACGCTA7100.013.9014099
GTTTATT100200.013.1337341
ATATACG10600.012.4150946
CGAAGAT84650.011.9929121
GTTTAAT81700.011.7931471
GTCGGTT48100.011.6278591
CGGAAAT41800.011.46891
GTTTTTT263350.011.4042161
GTTTTAT136200.011.3876651
GTTTTAC63100.011.2472271
CGAAAAT33950.011.213551
AAATCCA185250.011.1379224
CGCGTAT3802.5898727E-411.1315791
CGCAAAC41450.011.1121841
Produced by FastQC (version 0.11.5)