FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22452075
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104400.046.90996694
CAAGTCG80600.027.4069594
TAAGTCG30700.019.44299794
GAAGTCG120950.017.56428394
AAAGTCG45050.016.90122294
TACGCTA7650.014.7450989
TATACGC8250.013.6727297
GTTTTTT265650.012.15471651
GTTTATT98250.011.5287531
AATCGGG47950.011.4681965
CGAAAAT33850.011.3855251
GTCGGTT44600.011.38116551
GGAAAAT164000.011.3487811
GTTTTAT145950.011.335391
CTTTAAT97150.011.272261
GCGGTAT29500.011.1525431
CTTTATT153850.011.1504731
TTAGCGC19000.011.1315793
AATCGTA17750.011.1211285
CGAAGAT82300.011.078981
Produced by FastQC (version 0.11.5)