FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21831800
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG166450.051.1084494
CAAGTCG124000.028.23790494
TAAGTCG37350.024.66398894
GAAGTCG141650.022.46311494
AAAGTCG62550.017.88329394
GCCGAAT51300.015.3001961
GTTTTTT257350.014.7748191
CGCAAAC48950.014.3064351
GCGAAAT26750.013.8803731
GCGGAAT40800.013.47794151
GCGGTAT30450.012.9655171
CGCGAAT15600.012.9551291
CTCGATT40350.012.8128881
TTAGCGA16900.012.79297
AAATCCA181500.012.71464
AATCCAC111750.012.4071595
CGAAAAT56650.012.2789061
CTTTAAC66650.011.9174791
CGAATCC45500.011.8791213
GAAAAAT195350.011.8372161
Produced by FastQC (version 0.11.5)