FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23003505
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126050.040.94089594
CAAGTCG95400.023.594
TAAGTCG29350.022.73935394
CGCGTAA7600.017.3157881
GAAGTCG118600.016.44603794
TAGCGAG23000.011.0347829
GCGTAAC13200.010.6818192
GTCGTTT23800.010.4663881
TTAGCGA19100.010.3350798
AAAGTCG49600.010.328629594
TCGGATT25950.010.1425822
AATCGTA20600.010.0388355
TACCGTA21650.09.9861439
GGCGATT40100.09.9625941
CGGGAAT53000.09.93207551
ATATACG11900.09.873956
CGCGTAT5355.6789075E-59.6635511
CTCGGTT40300.09.5632741
CGTATCG18300.09.5027323
GTCGAAT14350.09.4982581
Produced by FastQC (version 0.11.5)