FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22218869
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG111150.056.74674294
CAAGTCG87100.026.98048494
TAAGTCG30650.022.84828894
GAAGTCG125000.018.98894
GTTTTTT290850.015.67474651
AAAGTCG49800.014.91164894
GCCGAAT46250.014.3286491
CGAAAAC30000.013.4733341
TTTTTTC366600.013.4358992
GAAAAAT218700.013.1307741
AAAAATC221150.012.8790432
GTCGATT21200.012.8584921
GCGAAAT29250.012.8547021
GTCGAAT14900.012.6174511
CGGATAT22400.012.5892861
CTCGATT37850.012.5416121
CGCAAAT28550.012.5113831
AAATCCA194550.012.3448994
CGCAATT19100.012.0575911
GGAAAAT173150.012.0519791
Produced by FastQC (version 0.11.5)