FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22218869
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG137600.045.3263194
CAAGTCG109250.023.8764394
TAAGTCG30550.022.15384994
GAAGTCG118900.018.02523294
GTTTTTT281250.014.1710221
GCGAAAT26000.014.11
GCCGAAT45250.013.2950291
AAAGTCG53700.013.21601694
CGAAAAC31700.013.1955841
CGCATAT9100.012.9120881
CTCGATT36250.012.5765521
AAATCGA33950.012.4594994
CGGAAAT43950.012.1911271
CGCGAAT12900.012.0232551
AAATCCA194050.011.3594454
CGCAAAT30300.011.3234331
AATCCAA107850.011.2433955
AAAAATC200750.011.2378592
GCGGAAT37900.011.1609511
TACGCTA6806.2827894E-911.0588249
Produced by FastQC (version 0.11.5)