FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22785220
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG107650.056.58337494
CAAGTCG98800.030.7307794
TAAGTCG26250.027.21523994
GAAGTCG117650.018.57628694
AAAGTCG44650.014.10526394
GTCGAAT13600.012.4411751
CGCGTAA10000.011.751
GGCGAAT64850.011.740941
GCGGTAT22900.011.6986891
AATCGTA20250.010.9086425
GTCCGAT13050.010.8045971
GCCGATT20650.010.6973361
CGTATCG13650.010.6739933
GTTCGAT32750.010.3328241
GTCGAAA32700.010.2048921
CGTAACG5305.123484E-59.7547173
GGGAAAT152000.09.7401311
GTCGGTT44400.09.7387381
CTCGGTT36650.09.6180091
GTCGGGT48350.09.526371
Produced by FastQC (version 0.11.5)