FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22785220
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG133650.045.5054394
CAAGTCG108300.028.07848594
TAAGTCG30400.020.25328894
GAAGTCG121000.018.528194
GCGGTAT24350.013.7043111
AAAGTCG49450.013.40141694
GGCGATT38250.011.4274521
CGCGTAA8701.8189894E-1211.3448291
GTCGTTT23050.011.214751
TCGGATT24450.010.9570562
TTAGCGA17500.010.7428578
GTCCGAT14600.010.301371
GTCGGTA16450.09.7142851
GTCGGTT43500.09.5080461
TAGCGAG22250.09.5056189
CGGGAAT54100.09.4695011
CGTATCG16400.09.4573173
CGCGTAT5003.0676674E-49.41
GGCGGTT56350.09.17481
CGGTATC34050.09.1101312
Produced by FastQC (version 0.11.5)