FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21929801
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG95950.046.68160294
CAAGTCG74850.024.2378194
TAAGTCG28300.020.75971894
GAAGTCG105750.016.894
GTTTTTT267050.015.9981291
TTTTTTC380700.015.086422
AAAGTCG43850.014.79133494
TACGCTA9000.014.6222229
TATACGC9150.013.8688527
GTTTAAT93150.013.7745571
TTTTTTG400350.013.7002632
AAATCGA33750.013.5081494
GTTTTAC66650.013.116281
CTTAGAT64000.013.0718761
AAAAATC201550.013.0354762
CGCAAAC41550.012.8953071
AATCGGG45700.012.855585
CGAAAAT36650.012.8240121
AAATCCA194250.012.5333334
CTCGATT44100.012.4693871
Produced by FastQC (version 0.11.5)