FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21929801
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126500.035.37075494
TAAGTCG29000.022.85172594
CAAGTCG87150.021.14056294
GAAGTCG111300.015.66666794
CTCGATT45500.015.5978021
GTTTTTT287100.014.88087751
GTTTAAT89400.014.2997761
AAATCCA190600.013.8336834
AAATCGA31350.013.7926644
CGCAAAC41650.013.4285721
GCGAAAT29400.013.2687071
AATCCAA104900.012.6348915
TATACGC9450.012.4338637
TACGCTA9850.012.4060929
CTCAGAT166450.012.1982571
GTTTTAC72150.012.1815671
CGAAAAC35250.011.8666671
AAAAATC191500.011.7806792
CGAACGT40350.011.764563
CTTAGAT65650.011.7410511
Produced by FastQC (version 0.11.5)