FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23474002
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101350.047.48692794
CAAGTCG76150.023.1451194
TAAGTCG31950.021.62441394
TATACGC7900.017.2531647
GAAGTCG123550.016.47187494
CGAATCG18250.014.4219183
ATATACG10350.013.6231886
CGAAAAT32500.013.0153851
TACGCTA9500.012.8631579
CGAAGAT92150.012.8019531
CGCATAT9200.012.7717391
GTTTATT100650.012.5613511
CGGAAAT48250.012.4683931
AAAGTCG45600.012.3684294
GTTTTTT284050.011.797571
TAAATCG10850.011.6958533
GTTTAAT93600.011.5993591
GTCGGTT52100.011.5470261
CGCGGAT29250.011.2478631
TCGGATC49700.011.0643852
Produced by FastQC (version 0.11.5)