FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21956940
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG93000.050.1333394
CAAGTCG72600.024.73002894
TAAGTCG26700.022.53183694
GAAGTCG110300.017.98186794
GTTTTTT295300.014.6745681
TTTTTTC383650.013.6840872
AAAGTCG47200.012.94491694
TTAGCGA19800.012.8181837
GCGAAAT27900.012.297491
CGAAAAT36150.012.0912871
GAAAAAT215800.012.0222431
TTTTTTG411650.011.9198352
AAATCCA191700.011.5477314
GTTTTAC65200.011.4616571
AATCGGG48800.011.4610665
GTTTTCT220650.011.43847751
GGAAAAT164000.011.4347561
CGAAAAC29750.011.374791
AAATCGA31600.011.3037974
AAAAATC206600.011.2153922
Produced by FastQC (version 0.11.5)