FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21956940
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG115750.040.80777794
TAAGTCG24950.024.300694
CAAGTCG85900.021.66705594
GAAGTCG117600.016.58588494
CGCGAAT7700.014.038961
GTTTTTT282800.013.6446261
CGAAAAT42800.013.2873831
AAAGTCG51800.012.33976894
GCGAAAT27100.012.1402221
CGCAAAC41600.011.8629811
AAAAATC197800.011.4767452
GTTTAAT85150.011.4257191
AAATCGA30150.011.3797674
CTCGATT36550.011.1874141
AATCCAA107700.011.1717745
GAAAAAT207400.011.1268081
AAATCCA190550.011.0254534
GGAAAAT161650.010.93226051
GTTTTAC66250.010.854341
AATCCAC111350.010.7633595
Produced by FastQC (version 0.11.5)