FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22181836
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG51750.017.61932494
GTTCGAT28300.012.7879861
GTCGTTT20750.011.7783141
TTAGCGA27050.011.29390058
GTCGGTT50400.011.28373051
TAGCGAG30000.011.2800019
CGTAACG6554.2826287E-810.7633593
TTCGATT31200.010.6955132
GTCGAAT13350.010.5617981
CGCGGAT24050.010.5530141
GTTTATT99100.010.0544911
TCGGATT25000.09.9642
AATCGTA18550.09.8814025
CGCAAAA34400.09.8372091
GTCCGAT15800.09.8164561
CTCGGTT38350.09.6818771
GGGAAAT159700.09.65311
TAAGTCG14750.09.55932294
CGGGAAT55450.09.4084761
GCGGTAT21500.09.41
Produced by FastQC (version 0.11.5)