FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22181836
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAT29600.013.1790531
TTAGCGA21800.013.1513768
TAGCGAG28300.012.2897539
AAGTCGG75800.011.84300794
GTCGTTT21700.011.6958521
TATACGG15650.011.56230134-35
CGTATCG16600.011.0421693
GGCGGAT88100.010.7763911
CGCGGAT25600.010.64843751
CGGGGTT96200.010.5041571
GTCCGAT14350.010.4808371
TTCGATT31050.010.4444442
CGGGAAT53000.010.1981131
CGCGAAT5103.357714E-510.1372551
TCGGATT25950.09.9614652
GGCGATT37500.09.9013341
CGCGTAA6353.608895E-69.6220471
CTCGGTT33750.09.6088891
CGTCGGA34800.09.58908194
GTATCGA22550.09.5875834
Produced by FastQC (version 0.11.5)