FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22003586
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG118400.036.7981494
CAAGTCG85650.022.55341394
TAAGTCG28700.019.65156794
CTCGATT54850.017.0519581
GTTTTTT296550.016.3877931
CGAAAAC39950.015.7647041
CGGATAT24250.015.1175241
GAAGTCG111550.015.08381894
CTTAGAT76900.014.1183361
AAATCCA201400.013.3718964
AAAAATC205500.013.3338182
CGCAAAC40900.013.2151591
TCGATTC60950.013.0319932
TACGCTA9850.012.8832479
TATACGC10650.012.7981217
CGAATCG16250.012.7261533
CGCGAAC13700.012.693431
CTTTAAC79700.012.6198241
CGAAAAT46650.012.5937831
GCGAAAT27650.012.5786611
Produced by FastQC (version 0.11.5)