FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21478864
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128500.057.78988694
CAAGTCG101650.030.60895394
TAAGTCG37900.027.77836694
GAAGTCG136750.023.92102494
AAAGTCG55950.018.81680194
CGCAAAT28500.016.491231
GTTTTTT287800.013.734191
TTTTTTC400800.012.5474062
CGAAAAT30300.012.4092421
CGGATAT22700.012.215861
CGCAATT22050.012.149661
GCGAAAT26750.012.1233651
GGAAAAT189850.012.0068491
GTCGATT19350.011.901811
CGGAAAT45250.011.633151
CGAATCG14300.011.5034963
CGCGTAT4951.9455874E-611.393941
GTTTTAC77000.011.3532471
TTTTTTG423150.011.2848872
GTTTTAT159000.011.2622641
Produced by FastQC (version 0.11.5)