FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21478864
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG147150.049.44342894
CAAGTCG114100.030.48203594
TAAGTCG37850.026.82166594
GAAGTCG132750.023.50885294
AAAGTCG62900.015.31796694
CGAAAAT34250.013.1737241
CGCGTAT4707.4040145E-813.0000011
GCGAAAT25900.012.88417151
GTTTTTT288950.012.8662411
CGGAAAT40000.012.221
GGAAAAT186950.011.4137471
GTTTAAT82700.011.1390561
TCGAATC21200.010.8632082
CTCAGAT162800.010.6240791
CGAATCG13850.010.5198563
GCGGTAT21250.010.3952941
AAATCCA192700.010.2682924
CGAAGAT70800.010.2231651
CTCGGAT58450.010.2121481
CGGATAT22650.010.1677711
Produced by FastQC (version 0.11.5)