FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22888736
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106600.054.7157694
CAAGTCG91000.029.07802294
TAAGTCG29050.024.26850194
GAAGTCG121600.019.0937594
GGCGAAT85900.013.2956921
AAAGTCG45450.012.92629294
AATCGTA18950.012.4010545
CGCGTAT5102.1465712E-711.9803921
GCGGTAT24650.011.2494931
CGAATGC83400.011.1019173
GTCGGTT41350.010.5707381
GCGAATG92200.010.5010842
GGCGATT35500.010.3267611
GGCGGTT53900.010.0278291
GTCGGAG46150.09.9804981
GGCGGAT91450.09.8162931
GGGAAAT161350.09.6126431
CGGTATA12750.09.5843132
GTTCGAT29650.09.5109611
GTCGATT22550.09.3791571
Produced by FastQC (version 0.11.5)