FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22373035
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131850.035.1118794
TAAGTCG29050.024.75387494
CAAGTCG104100.020.9039494
GAAGTCG112750.015.96541194
GTAAAAC62000.013.26612951
CGAAGAT75700.012.35535051
CGCAATT21350.012.3278681
GGAAAAT153650.012.2661891
AATCGGG36800.011.8777175
GTTTAAT80550.011.669771
CGAAAAT49350.011.4285721
AAATCGA29750.011.374794
CGCTAAT10000.011.281
GTTTTTT275900.011.2091341
GTCGGTT40900.010.9168691
CGCAAAT33200.010.9006031
CGCGTAT4751.5317764E-510.8842121
CTTTAAT95800.010.8423811
GTTTATT99500.010.769851
CGCAAAC44300.010.7155751
Produced by FastQC (version 0.11.5)