FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22978764
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG101200.047.7430894
CAAGTCG81450.026.19766694
TAAGTCG27450.024.48451694
GAAGTCG115350.018.57997594
GTTCGAT29350.015.2129461
AAAGTCG44100.013.21541994
GTCCGAT14850.012.6599321
GCGGTAT22050.011.9365071
TAGCGAG31200.010.8461539
GTCGGTT49500.010.7292921
GTTTATT100300.010.4496511
TTCGATT35100.010.4444452
TCGGATT25700.010.4241242
CGCGTTT13600.010.3676461
TTAGCGA27400.010.2919718
CGTATCG18400.010.2173913
CGCGGAT23850.010.0503141
CGCGTAT5153.7381178E-510.0388351
GTCGAAT13600.010.02205851
GTCCGTT24450.09.995911
Produced by FastQC (version 0.11.5)