FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23104967
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG96950.050.4177494
CAAGTCG84950.027.60800494
TAAGTCG30300.022.33663294
GAAGTCG110950.016.0973494
AATCGTA23750.013.65473655
CGCGGAT25000.013.161
TTAGCGA22850.012.5470458
GTTCGAT26000.012.1115381
GTCCGAT15850.011.8611971
GGCGAAT61650.011.740471
ATCGTAT17650.011.1841356
GCGGTAT22350.010.93512251
TAGCGAG31150.010.8635639
AAAGTCG46350.010.85005394
GTCGAAA33150.010.6334841
CGTATCG19250.010.2545443
CGCGTAT5053.0125779E-510.2376231
GTCGGGT45550.010.1119641
GTCGCTT29750.010.1109251
GTCGGAA37250.010.0939591
Produced by FastQC (version 0.11.5)