FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23104967
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG119500.040.94309294
CAAGTCG97400.026.0092494
TAAGTCG30100.021.07973594
GAAGTCG114800.014.329267594
GGCGAAT51350.012.6309631
GTCGTTT23500.012.5999981
TTAGCGA22850.012.5470458
GCGGTAT23050.012.4381781
CGCGTAT4355.7632915E-611.88505651
GTTCGAT26100.011.3448271
AATCGTA21900.010.9452065
GGCGGAT89250.010.8481791
GGGAAAT157350.010.5738791
TAGCGAG29950.010.3572629
GTCGGGT45400.010.3524221
GCGTAAT7352.1114829E-810.2312921
ATCGTAT18200.010.0714286
GTCCGAT16800.010.0714271
CGTATCG18950.09.9208443
GTCGTGT38450.09.9011711
Produced by FastQC (version 0.11.5)