FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23142748
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG92750.045.3024394
TAAGTCG31450.022.86486694
CAAGTCG66800.022.5149794
TACGCTA11100.021.5945939
TATACGC12900.018.2170547
GAAGTCG108850.016.79650994
CCAATCG29600.016.5135143
GTTTTTT318850.015.9639331
ATATACG16350.015.8103986
CAATCGG37350.014.8487274
TTTTTTC443650.014.3971612
CGAAAAC42100.014.2897871
CGCGAAT8600.014.2093011
GTTTTAC80750.014.0272451
CTCGATT52300.013.6596561
AATCGGG53450.013.4536955
TTTTTTG473450.013.36191752
CGAAAAT39950.013.1764711
ATCGGGT37000.013.0837836
TCGGGTA35950.013.0737137
Produced by FastQC (version 0.11.5)