FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23142748
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127550.033.23716494
CAAGTCG89500.021.00558794
TAAGTCG32050.015.98439994
TATACGC11550.015.4632037
GAAGTCG110800.014.8465794
GTTTTTT313850.014.4062451
CGAAAAC46900.014.330491
TACGCTA12350.013.7004059
GTTTTAC79150.013.1825651
GTCGATT19250.012.940261
CGCGAAT8400.012.8690471
GTTTAAT101950.012.8621881
GGAAAAT178900.012.4790381
CTTAGAT78700.012.4218561
AAATCCA209500.012.3164674
AAAAATC232850.012.1511712
CGCAAAC44100.012.1496591
CGCGAAC15100.012.1390731
CTCAGAT168650.012.0670021
CAATCGA21900.012.0182654
Produced by FastQC (version 0.11.5)