FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22098021
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG55500.032.8576694
GTTTATT116500.015.1690991
CGAAAAT39600.015.0732321
CAAGTCG44300.014.95936794
CGCAAAT27900.013.8136211
GTTCGAT28850.013.6845751
GTCGGTT55850.013.6329461
GTTTAAT112800.012.9583331
GTTTTAT178200.012.8709321
CGCGGGT48450.012.5139311
GTTTGAT140900.012.37544251
GTCAATT68700.012.314411
GTTTTTT346100.012.2354811
GTCGATT21750.012.1011491
CTTTATT185200.012.0545361
GTCAAAT84350.012.0355661
GGAAAAT198750.011.9657861
CGAAGAT102650.011.904531
CTCGTTT39050.011.7951351
CGCAAAC39600.011.7499991
Produced by FastQC (version 0.11.5)