FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22098021
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG80150.023.22145894
CGCAAAT29550.015.9052461
CGCGTAT4802.5465852E-1115.6666671
CTCGTTT39500.013.802531
CGAATCG12600.013.8015883
CGCGTTT12950.013.4285721
GTTTATT128200.013.1981281
CGCAAAC41600.012.9927881
GTTCGAT29400.012.9489791
GTTTTAT169400.012.76269151
GTTTAAT106700.012.5979391
CGCGTGT20900.012.5933011
CACGCGT17550.012.3190889
GTTTTTT335250.012.2669651
CGCGATT5756.2482286E-912.2608691
CTTTATT191900.012.1724851
GTCAATT71800.011.913651
TAAGTCG24350.011.77412894
GTTTTCT242000.011.7305781
GTTTGAT121650.011.5519931
Produced by FastQC (version 0.11.5)