FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21020161
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG69400.042.3948194
CAAGTCG52150.020.9990494
TAAGTCG23300.018.75965594
GAAGTCG87800.016.21981694
CGCTTAA37950.013.18972244-45
AATCGTA25500.012.1647055
CGTTCGA14150.011.9575977
TCGGATT24800.011.9395152
GTCGGTT37300.011.3404821
GTCGAAT16250.011.281
GGCGAAT41650.011.0588231
CGCGGAT19300.010.7150251
TAATCGT23300.010.6909874
ACGTTCG17350.010.564846
ATCGTAT20950.010.5441526
CGCGTAA8401.5315891E-910.0714281
CACGCTT51400.09.96692542-43
ATAATCG23150.09.9481643
GTCGGAT25150.09.9045721
GTTCGAT25300.09.8458491
Produced by FastQC (version 0.11.5)