FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22229276
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131400.058.7321294
CAAGTCG113350.035.28628594
TAAGTCG32850.026.03957694
GAAGTCG131700.022.01898294
AAAGTCG47550.017.09989594
CGTAACG4201.6925696E-814.5476193
GTTCGAT32700.013.5107041
GTCCGAT15600.012.9551291
TATACGG17450.012.92836734-35
GTCGAAT13350.012.3220991
TTAGCGA21200.012.1933978
GCGGTAT22000.012.1772741
TTCGATT36850.011.9891462
GTCGTAT9900.011.8686881
GGCGATT40500.011.8370381
GTCGGTT46450.010.9278791
GTCGGAA40500.010.9086431
CGTTCGA19400.010.9020627
GCAAGTC122650.010.72971994
GGGAAAT165850.010.457041
Produced by FastQC (version 0.11.5)