FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22208507
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG153350.048.91555494
CAAGTCG118600.028.6517794
TAAGTCG40100.026.72319294
GAAGTCG140750.022.57335794
CGAGAAC71200.014.390451
AAAGTCG65000.013.73846194
CGAAAAT40400.013.6113851
CTCGATT32750.013.0595421
GTTTTTT300000.012.7683331
CGAAAAC39000.012.6538461
CGAAGAT79200.012.5214651
CGCAAAT30300.012.09900951
CGAGAAT38550.012.0700391
CGCGTTT14500.011.9931031
CTCGTTT40150.011.5890421
AAATCGA37850.011.5482174
TCGCAAA36700.011.397819514-15
CTTTAAT132550.011.169371
GGAAAAT192850.010.9670731
GTCGATT17250.010.8985511
Produced by FastQC (version 0.11.5)