FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21954566
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG92050.048.0467194
CAAGTCG73500.024.61904794
TAAGTCG29400.022.54081794
GAAGTCG102100.020.43878494
CGAAAAT37500.017.0453341
GTTTTTT296550.015.8647781
TTTTTTC381850.014.9302082
TTTTTTG426950.013.1769522
AAAGTCG47100.013.072185594
GAAAAAT232900.012.8952341
CGCGAAT20550.012.8077851
AAATCCA206850.012.7469184
GTTTTAT164800.012.719661
GTTTAAT98000.012.7091831
GTTTTAC66750.012.6741561
GTTTTTC164850.012.4592051
TTTTTTA408050.012.2783972
GTCGATT18000.012.011111
AATCCAC120650.011.9983425
CGGATAT23900.011.9958141
Produced by FastQC (version 0.11.5)