FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21954566
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114850.038.54940894
TAAGTCG28250.024.12389294
CAAGTCG94850.020.3658494
GAAGTCG105300.018.03228894
GTTTTTT320300.014.6590681
CGCAAAT32250.014.4279071
AAATCGA32650.013.9632464
AAAGTCG50900.013.94302594
TATACGC7550.013.6953637
AAATCCA199700.013.0620924
CGGATAT21750.012.9655171
TCGAATC19650.012.9160292
CGAAAAT38300.012.8851181
CTCGATT39150.012.8454661
TACGCTA7800.012.6538469
AAAAATC210300.012.5601522
AATCCAA110400.012.5163045
GTTTAAT93900.012.5133121
CGAAAAC31750.012.4346451
CGCAATT19450.012.3239071
Produced by FastQC (version 0.11.5)