FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23140858
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG98200.051.25967894
CAAGTCG76300.024.94757794
TAAGTCG31650.021.82938494
GAAGTCG112150.017.64333594
GTTCGAT24000.014.6875021
GTCCGAT16500.012.8181821
AATCGTA26650.011.6397755
CGCTTAA35050.011.59914444-45
GTCGGAA36750.011.5102041
CGACCTA11350.011.1806164
CGCGTAA5901.17293894E-711.1525431
CGCGGAT20900.011.0191391
GTCGTTT23950.010.9895621
CTCGGAT60750.010.9860091
TATACGG15900.010.78930934-35
TTCGATT31200.010.5448722
AAAGTCG48150.010.54205694
GTCGGTT54300.010.4732971
TCGGATT31000.010.4612912
GGCGATT42700.010.1264641
Produced by FastQC (version 0.11.5)