FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21945831
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114600.051.0602194
CAAGTCG84850.028.80377494
TAAGTCG32550.026.42396494
GAAGTCG129650.018.814594
TATACGC8800.017.090917
TACGCTA9100.016.5274739
ATATACG11950.016.518836
TTAGCGA28950.015.0984467
TAGCGAG32450.014.1941438
GTCGGTT46000.014.0999991
AAAGTCG48200.013.74896394
CGGAAAT49700.013.144871
TTTTTTC334650.012.9630972
GTTTATT96650.012.4490431
AATCGGG61750.012.2542515
GTTTTTT281000.011.9590741
GTTTTAC58450.011.5791281
CGAATCG15450.011.5598713
CGAAAAT32200.011.5310551
CTTTATT158200.011.3489251
Produced by FastQC (version 0.11.5)