FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21945831
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG146000.037.6321994
CAAGTCG97950.024.6156294
TAAGTCG31400.022.00318594
GAAGTCG128100.018.45511294
TATACGC9700.016.9587617
TACGCTA10600.014.6320759
ATATACG11700.014.4615386
TTAGCGA19700.014.0761427
CGAAGAT82600.013.4854741
CGAAAAT45450.012.8228831
GGAAAAC135450.012.24881
AATCGGG49200.011.9410565
AAAGTCG57100.011.68826694
CGCAATT22800.011.54385951
GCGAAAT29600.011.4324331
GTCGGGT60600.011.400991
GCGGTAT37900.011.1609511
CGCAAAC41500.011.0987951
GTAAAAC63350.011.054461
GGAAAAT157200.011.0025431
Produced by FastQC (version 0.11.5)